TRP2/DCT Antibody (C-9) | SCBT - Santa Cruz Biotechnology (2024)

• TRP2/DCT Antibody (C-9) is a mouse monoclonal IgG_2a κ TRP2/DCT antibody, cited in 51 publications, provided at 200 µg/ml
• raised against amino acids 41-190 of TRP2 of human origin
• TRP2/DCT Antibody (C-9) is recommended for detection of TRP2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
• Anti-TRP2/DCT Antibody (C-9) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
• also available conjugated to Alexa Fluor^® 488, Alexa Fluor^® 546, Alexa Fluor^® 594 or Alexa Fluor^® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
• also available conjugated to Alexa Fluor^® 680 or Alexa Fluor^® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR^®Odyssey^®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
• Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of TRP2/DCT (C-9): sc-74439.
• m-IgG Fc BP-HRP, m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for TRP2/DCT Antibody (C-9) for WB and IHC(P) applications. These reagents are now offered in bundles with TRP2/DCT Antibody (C-9) (see ordering information below).

TRP2 Antibody (C-9) is an IgG_2a κ mouse monoclonal TRP2 antibody (also designated DCT antibody, or dopachrome tautomerase antibody) that detects the TRP2 protein of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA. TRP2 Antibody (C-9) is available as both the non-conjugated anti-TRP2 antibody form, as well as multiple conjugated forms of anti-TRP2 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor^® conjugates. Tyrosinase (TYR), a type I membrane protein and copper-containing enzyme, is involved in the production of melanin, the primary pigment found in vertebrates. Melanin biogenesis requires the enzymatic activity of TYR, which catalyzes the critical and rate-limiting step of tyrosine hydroxylation in the biosynthesis of melanin. Defects effecting TYR activity result in various forms of albinism. The TYR-related proteins, TRP1 and TRP2, are also specifically expressed in melanocytes, and they likewise contribute to the synthesis of melanin within the melanosomes. The TRPs, including TYR, all share a similar transmembrane region, contain two metal-binding regions and a cysteine-rich epidermal growth factor motif, and are localized in the melanosomal membrane. These proteins, however, have distinct catalytic activity, and they individually contribute to the biosynthesis of melanin biopolymers. The TRPs are believed to exists as a multi-enzyme complex, as these proteins form aggregates together, and the expression of TRP1 also helps stabilize TYR in melanocytes.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

TRP2/DCT 抗体 (C-9) References:

1. Mutational mapping of the catalytic activities of human tyrosinase. | Tripathi, RK., et al. 1992. J Biol Chem. 267: 23707-12. PMID: 1429711
2. A second tyrosinase-related protein, TRP-2, is a melanogenic enzyme termed DOPAchrome tautomerase. | Tsukamoto, K., et al. 1992. EMBO J. 11: 519-26. PMID: 1537333
3. Cloning and expression of cDNA encoding mouse tyrosinase. | Shibahara, S., et al. 1986. Nucleic Acids Res. 14: 2413-27. PMID: 3008090
4. Mammalian tyrosinase--the critical regulatory control point in melanocyte pigmentation. | Hearing, VJ. and Jimεnez, M. 1987. Int J Biochem. 19: 1141-7. PMID: 3125075
5. Mammalian tyrosinase catalyzes three reactions in the biosynthesis of melanin. | Körner, A. and Pawelek, J. 1982. Science. 217: 1163-5. PMID: 6810464
6. High-molecular-weight forms of tyrosinase and the tyrosinase-related proteins: evidence for a melanogenic complex. | Orlow, SJ., et al. 1994. J Invest Dermatol. 103: 196-201. PMID: 8040609
7. Molecular characterization of a human tyrosinase-related-protein-2 cDNA. Patterns of expression in melanocytic cells. | Bouchard, B., et al. 1994. Eur J Biochem. 219: 127-34. PMID: 8306979
8. Molecular interactions within the melanogenic complex: formation of heterodimers of tyrosinase and TRP1 from B16 mouse melanoma. | Jimεnez-Cervantes, C., et al. 1998. Biochem Biophys Res Commun. 253: 761-7. PMID: 9918801